Journal: JID Innovations

Article Title: CIT tumor lines: A series of immunogenic murine cutaneous squamous cell carcinoma cell lines derived from chemical carcinogenesis

doi: 10.1016/j.xjidi.2026.100477

Figure Lengend Snippet: CIT lines express identifiable neoantigens whose cognate CD8+ T cells are responsive to immunotherapy. ( a ) Selection criteria for CIT6 mutations predicted to generate neoantigens. (b) Predicted peptide log affinity ratio (affinity ratio is defined as the ratio between predicted binding affinity of mutant versus wild-type sequence) and gene expression level of mutations predicted to be top CIT6 candidate neoantigens, shown for H2-Dq and H2-Kq alleles. Affinity predictions were generated using the NetH2Pan algorithm. (c) Schematic of IFNγ ELISpot assay. For screening of CD8+ T cell and CD4+ T cell responses, CD8+ or CD4+ T cells were isolated from the spleens of tumor-bearing mice and co-cultured with total splenocytes from a healthy tumor-naïve mouse. (d) Representative IFNγ ELISpot images of the assay performed with CD8+ T cells from the spleen of CIT6-bearing mice cultured with 160ug/ml of Brinp3 peptide CPAFLPCTV (top row) and negative control (no peptide, bottom row). (e) Quantification of average IFNγ ELISpot spot counts for CD8+ T cells from the spleen of CIT6-bearing mice cultured with different concentrations of Brinp3 peptide CPAFLPCTV. Each colored line represents data points from a different mouse (n = 4 mice). (f) Representative IFNγ ELISpot images of the assay performed with CD8+ T cells from the spleen of CIT6-bearing mice cultured with 160ug/ml of Ttll4 peptide VPPSSLLPL (top row) and negative control (no peptide, bottom row). (g) Quantification of average IFNγ ELISpot spot counts for CD8+ T cells from the spleen of CIT6-bearing mice cultured with different concentrations of Ttll4 peptide VPPSSLLPL. Each colored line represents data points from a different mouse (n = 4 mice). (h) Spot counts from ELISpot assays performed with CD4+ T cells isolated from the spleens of CIT6-bearing mice and cultured with splenocytes from tumor-naïve mice loaded with increasing concentrations of Brinp3 and Ttll4 peptides. Each colored line represents data points from a different mouse (n = 4 mice). (i) Spot counts from ELISpot assays performed with total splenocytes from CIT6-bearing mice cultured with increasing concentrations of Brinp3 and Ttll4 peptides. Each colored line represents data points from a different mouse (n = 10 – 11 mice). (j) Schematic of ICI treatment regimen and ELISpot analysis. (k) Average spot counts of ICI-treated splenocytes cultured with 80 ug/ml of Ttll4 peptide vs negative control. Each line represents data points from a different mouse (n = 6 mice). Statistical analysis in panels e, g, h, i, and k was done by a paired t- test. ∗ P < .05, ∗∗ P < .01. Error bars in panels e, g, h and i represent mean and SD. CIT, carcinogen-induced tumor; ICI, Immune checkpoint inhibitor.

Article Snippet: Eight-week-old wild-type FVB/N mice were purchased from Jackson Laboratories.

Techniques: Immunopeptidomics, Selection, Binding Assay, Mutagenesis, Sequencing, Gene Expression, Generated, Enzyme-linked Immunospot, Isolation, Cell Culture, Negative Control

Journal: bioRxiv

Article Title: Characterization of tumour interactions with the immune system in an autochthonous mouse model of glioblastoma

doi: 10.64898/2026.05.13.724869

Figure Lengend Snippet: Neonatal FVB/N mice were injected without using the adaptor (red) or with the adaptor (green) and euthanized at humane endpoint. Median survival times were 85 days and 44 days (P = 0.011 by Log Rank test). For the mice injected without the adaptor, one of two surviving mice showed the presence of tumour after euthanasia; for the mice injected with the adaptor, all remaining mice showed tumours . B. Example of late stage tumor. The hemisphere where the lateral ventricle injection was performed is shown at the top (large red arrow). Extensive invasion along the corpus callosum into the opposite hemisphere (small red arrow) is evident. C. Hemorrhaging and necrosis in tumour at humane endpoint . A different section from the same mouse as C is shown. D. Palisading necrosis. A close up of the section shown in C is shown (area marked by red box). E. Example of an early stage tumor detected by fluorescence microscopy for GFP. LV, lateral ventricle.

Article Snippet: Pregnant FVB/N mice were from Charles River.

Techniques: Injection, Fluorescence, Microscopy

Journal: bioRxiv

Article Title: Characterization of tumour interactions with the immune system in an autochthonous mouse model of glioblastoma

doi: 10.64898/2026.05.13.724869

Figure Lengend Snippet: A. The left panel shows a whole brain coronal section from a normal FVB/N mouse stained for TGFB1 expression; the right panel show the same for an SB model tumor-bearing mouse. B. The left panel shows a close up of the necrotic region indicated by the red box in A. The right panel shows the same region in an adjacent section stained for Iba1. C. Close up of leptomeninges and brain parenchyma in a normal FVB/N mouse (left panel) and an SB model mouse (right panel) stained for TGFB1. Images were taken at matched higher contrast than used in A to emphasize the diffuse expression of TGFB1 in brain parenchyma.

Article Snippet: Pregnant FVB/N mice were from Charles River.

Techniques: Staining, Expressing